Channel Title: Applied Biological Materials - abm Channel Description: Subscribe to our channel for educational videos covering essential topics in cell biology and life sciences. From practical "How-to" videos for those ready to get started in the lab to videos introducing basic principles of important new scientific techniques. Send us your requests by posting a comment! Applied Biological Materials (abm) provides quality research reagents and custom services at incredible prices. We have an extensive product range to make abm your one-stop supplier for all your research needs: PCR, RT-PCR, qPCR, antibodies, siRNA, miRNA, CRISPR, stable cell lines, immortalized cell lines, growth factors, and various viral expression systems (adenovirus, lentivirus, AAV). Conducting research shouldn’t cost you a fortune! abm is here to help! → Our company website: https://www.abmgood.com/ → Our FREE learning resources: https://info.abmgood.com/learning-resources #SciComm #CellBiology <video> RNA Mango Technology #shorts Description: Check out the RNA Mango technology in action! Developed by Dr. Peter Unrau and his team at Simon Fraser University, RNA Mango is a breakthrough advancement in RNA tracking, visualization and pull down technology. Visit our website for more information! Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences: ➜ Facebook: https://www.facebook.com/appliedbiological.materials/ ➜ Twitter: https://twitter.com/abm_good ➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material ➜ Instagram: https://www.instagram.com/abm_good ➜ Blog: https://info.abmgood.com/blog </video> <video> Adenovirus Production: How to Clone, Package, and Harvest Adenovirus for Your Seed Stocks Description: Adenoviruses are non-integrating and can deliver large transgenes (up to 6 kb) to a broad range of host cells (both dividing and non-dividing) with high efficiency, providing excellent yields of recombinant protein. In this video, we'll explain how to package second generation adenoviruses and generate high titer seed stocks! Here are some helpful time stamps for our adenovirus packaging video: 1:50 How to clone your gene of interest into an adenovirus expression system. We recommend abm's Adeno-1 to Adeno-8 cloning kits to easily speed up this process! https://www.abmgood.com/Adenovirus-Expression-Systems.html 3:07 How to amplify and linearize your recombinant adenovector for use in the transfection step. 3:41 Preparing your HEK293 cells for transfection (a great adenovirus packaging cell line). 4:21 How to prepare your transfection solutions. We recommend abm's DNAfectin™ Plus transfection reagent (Cat. No. G2500). https://www.abmgood.com/dnafectin-plus-transfection-reagent-g2500.html 5:45 How to transfect your HEK293 cells. 6:15 Checking your cells for successful transfection 6:32 How to make your adenovirus seed stock and amplify it to achieve a higher titer! 7:00 How to harvest and store your adenoviruses. Learn more about the recombinant adenovirus system at our learning resources center: https://info.abmgood.com/adenovirus-system Browse our collection of ready-to-use adenoviruses for every human, mouse, and rat gene: https://www.abmgood.com/Adenovirus.html We also offer ready-to-use CRISPR knockout adenoviruses targeting every human, mouse, or rat gene: https://www.abmgood.com/CRISPR-Knockout-Adenovirus-Library.html Need a custom recombinant adenovirus? We can make it for you! Simply tell us your project details and we'll do the gene synthesis, cloning, and adenoviral packaging for you: https://www.abmgood.com/Custom-Recombinant-Adenovirus-Service.html Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences: ➜ Facebook: https://www.facebook.com/appliedbiological.materials/ ➜ Twitter: https://twitter.com/abm_good ➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material ➜ Instagram: https://www.instagram.com/abm_good ➜ Blog: https://info.abmgood.com/blog </video> <video> What is Cell Transfection? #shorts Description: "What is cell transfection? Did you know that there are many considerations for a successful transfection experiment? Check our full video to find out 🙌 🔗 Link to our Youtube video: https://youtu.be/FJahRgP21a4" Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences: ➜ Facebook: https://www.facebook.com/appliedbiological.materials/ ➜ Twitter: https://twitter.com/abm_good ➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material ➜ Instagram: https://www.instagram.com/abm_good ➜ Blog: https://info.abmgood.com/blog </video> <video> How to Screen for CRISPR Knock Out Cells Description: After successfully using CRISPR to genetically modify your cells, you're ready to move on to the next phase of your project! But how can you ensure that your CRISPR knockout worked as intended? In this video, you'll learn how to confirm a complete knockout and explore three potential strategies to screen your CRISPR knockout clones. Here are some helpful time stamps for our CRISPR Cas9 Screen It™ Cleavage Detection Kit (Cat. No. G990) video: 0:15 Introduction 2:21 Sanger Sequencing 3:03 Mismatch Cleavage Assay 5:54 In vitro Cas9 Cleavage Detection Assay 7:34 Summary 8:43 Thank You! Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences: ➜ Facebook: https://www.facebook.com/appliedbiological.materials/ ➜ Twitter: https://twitter.com/abm_good ➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material ➜ Instagram: https://www.instagram.com/abm_good ➜ Blog: https://info.abmgood.com/blog </video> <video> Cell Culture Contamination and Quality Control #shorts Description: You're in the middle of preparing your cell cultures for an experiment but wait a minute - is that contamination? If so, who's responsible? Cell culture contamination is a pain but if it's not dealt with it can become an even bigger headache. Check out our YouTube video where we'll help you identify the calling cards left behind by the most notorious cell culture culprits: bacterial contamination, fungal and yeast contamination, mycoplasma contamination, and cell line cross-contamination! Link to the full video: https://youtu.be/tuJ8VWvX01M?si=NL9-dY9vTo3kbKO7 Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences: ➜ Facebook: https://www.facebook.com/appliedbiological.materials/ ➜ Twitter: https://twitter.com/abm_good ➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material ➜ Instagram: https://www.instagram.com/abm_good ➜ Blog: https://info.abmgood.com/blog </video> <video> abmVacations+ Rewards Program Description: Take a break from labwork - join our abmVacations+ Program and go on your dream vacation! How does the program work? • Earn points for every dollar spent on any Applied Biological Materials (abm) purchases of life sciences reagents or services on our online store: https://www.abmgood.com/ What can I get with my points? • Exchange your points for: →Trip for 2 to Las Vegas →Trip for 2 to Cancun →Trip for 2 to Osoyoos (British Columbia) →Movie Tickets →VISA Gift Cards →Coffee Cards How do I join? • Simply create an abm account and start earning points with your orders! Learn more about our abmVacations+ program: → https://www.abmgood.com/rewards-program.html#abmvacations </video> <video> Introduction to Cell Transfection: Part 1 Description: Today we will be exploring the cell culture technique, Transfection. In this video, we will help you learn what transfection is, its application, ways to perform transfection, and go over a quick guideline for your consideration when preparing for your experiments! Here are some helpful time stamps for our Transfection video: 0:28 What is Transfection? 1:31 Applications 2:54 Consideration - What do you want to do? 3:29 Consideration - Knowing your cells to determine experimental conditions 4:31 Consideration - Plasmid Design 5:11 Consideration - Type of Transfection 6:14 Thank You! Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences: ➜ Facebook: https://www.facebook.com/appliedbiological.materials/ ➜ Twitter: https://twitter.com/abm_good ➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material ➜ Instagram: https://www.instagram.com/abm_good ➜ Blog: https://info.abmgood.com/blog </video> <video> Cre-Lox Recombination Description: Did you know that tissue-specific expression in mouse models is easy to achieve with a DIO switch? Learn about Cre-Lox Recombination with our video! 🔗 Link to the full video: https://youtu.be/I21NmFq4F8A?si=1HuUC6FQ0bQrG8pL Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences: ➜ Facebook: https://www.facebook.com/appliedbiological.materials/ ➜ Twitter: https://twitter.com/abm_good ➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material ➜ Instagram: https://www.instagram.com/abm_good ➜ Blog: https://info.abmgood.com/blog </video> <video> High-titer Adenovirus and Titration Description: Recombinant Adenoviruses are wonderful tools used by the scientists for high efficiency, large gene sequence transfers into almost any cell type! In this video, we'll help you how to perform high-titer viral amplification with adherent or suspension HEK 293 cells, virus purification using iodixanol or Cesium chloride density gradient ultracentrifugation, and virus titration by end-point dilution assay. Here are some helpful time stamps for our adenovirus purification & titration video: 1:42 High-titer viral amplification with HEK293 cells 4:34 High titer Virus Purification 4:37 Purification by iodixanol gradient ultracentrifugation 6:25 Purification by cesium chloride (CsCl) density gradient ultracentrifugation 7:57 Virus Titration by end-point dilution assay Watch our video "Adenovirus Production: How to Clone, Package, and Harvest Adenovirus for Your Seed Stocks": https://youtu.be/8PEQpk7XfiU Learn more about the recombinant adenovirus system at our learning resources center: https://info.abmgood.com/adenovirus-system Browse our collection of ready-to-use adenoviruses for every human, mouse, and rat gene: https://www.abmgood.com/Adenovirus.html We also offer ready-to-use CRISPR knockout adenoviruses targeting every human, mouse, or rat gene: https://www.abmgood.com/CRISPR-Knockout-Adenovirus-Library.html Need a custom recombinant adenovirus? We can make it for you! Simply tell us your project details and we'll do the gene synthesis, cloning, and adenoviral packaging for you: https://www.abmgood.com/Custom-Recombinant-Adenovirus-Service.html Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences: ➜ Facebook: https://www.facebook.com/appliedbiological.materials/ ➜ Twitter: https://twitter.com/abm_good ➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material ➜ Instagram: https://www.instagram.com/abm_good ➜ Blog: https://info.abmgood.com/blog </video> <video> Introduction to Cell Transfection: Part 2 Description: Welcome to "Introduction to Cell Transfection: Part 2" video! Are you ready to dive deeper into the world of transfection? Let's explore what transfection is, its application, methods to perform transfection, and go over a quick guideline for your consideration when preparing for your experiments. Here are some helpful time stamps for our Transfection Methods video: 0:17 Calcium Phosphate Transfection 0:42 Simple Guide in Protocol (Calcium Phosphate Transfection) 2:08 Advantages and Disadvantages (Calcium Phosphate Transfection) 2:54 Lipid - Mediated Transfection 3:30 Simple Guide in Protocol (Lipid - Mediated Transfection) 4:47 Advantages and Disadvantages (Lipid - Mediated Transfection) 5:36 Physical Transfection 6:16 Simple Guide in Protocol (Physical Transfection) 7:29 Advantages and Disadvantages (Physical Transfection) 8:34 Summary 8:58 Thank You! Featured Product Links: Susfectin™ (Cat. No. G4000): https://www.abmgood.com/susfectin-transfection-reagent.html DNAfectin™ Plus (Cat. No.G2500): https://www.abmgood.com/dnafectin-plus-transfection-reagent-g2500.html Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences: ➜ Facebook: https://www.facebook.com/appliedbiological.materials/ ➜ Twitter: https://twitter.com/abm_good ➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material ➜ Instagram: https://www.instagram.com/abm_good ➜ Blog: https://info.abmgood.com/blog </video> <video> What is PCR and How Does It Work? #shorts Description: As the coronavirus that causes the COVID-19 disease spreads across the world, health organizations use real-time PCR, one of the most accurate laboratory methods for detecting the virus. So, what is PCR and how does it actually work? Check out the full video here: https://youtu.be/matsiHSuoOw?si=VNi1qBHBzKgWJph Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences: ➜ Facebook: https://www.facebook.com/appliedbiological.materials/ ➜ Twitter: https://twitter.com/abm_good ➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material ➜ Instagram: https://www.instagram.com/abm_good ➜ Blog: https://info.abmgood.com/blog </video> <video> Cell Culture Contamination and Quality Control Description: You're in the middle of preparing your cell cultures for an experiment but wait a minute - is that contamination? If so, who's responsible? Cell culture contamination is a pain but if it's not dealt with it can become an even bigger headache! In this video, we'll help you recognize the different calling cards left behind by the most notorious cell culture criminals: bacterial contamination, fungal and yeast contamination, mycoplasma contamination, and cell line cross-contamination. If contamination in your cell cultures is caught early, you might still be able to clean up the mess left by the culprits and save your precious cell cultures. ➜ Read more about how to detect and eliminate cell culture contamination in our "Cell Culture Quality Control and Contamination" article: https://info.abmgood.com/cell-culture-quality-control-contamination. Request your free sample of: • Mycoplasma PCR Detection Kit (Cat. No. G238): https://www.abmgood.com/pcr-mycoplasma-detection-kit-g238.html • Mycoplasma Elimination Cocktail (Cat. No. G398): https://www.abmgood.com/mycoplasma-elimination-cocktail-g398.html Learn more about our Cell Line Authentication Service: https://www.abmgood.com/Custom-Cell-Authentication-Service.html Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences: ➜ Facebook: https://www.facebook.com/appliedbiological.materials/ ➜ Twitter: https://twitter.com/abm_good ➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material ➜ Instagram: https://www.instagram.com/abm_good ➜ Blog: https://info.abmgood.com/blog </video> <video> Lentivirus Transduction Protocol #shorts Description: Having trouble with lentiviral infection of your target cells? Watch our step-by-step protocol for adherent and suspension cell types, in addition to using transduction enhancer to boost efficiency! Check out the full video here: https://youtu.be/LVsBMm0rX-c?si=Hf1y_zUQlq18x3Z7 Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences: ➜ Facebook: https://www.facebook.com/appliedbiological.materials/ ➜ Twitter: https://twitter.com/abm_good ➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material ➜ Instagram: https://www.instagram.com/abm_good ➜ Blog: https://info.abmgood.com/blog </video> <video> Applied Biological Materials - abm Live Stream Description: Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences: ➜ Facebook: https://www.facebook.com/appliedbiological.materials/ ➜ Twitter: https://twitter.com/abm_good ➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material ➜ Instagram: https://www.instagram.com/abm_good ➜ Blog: https://info.abmgood.com/blog </video> <video> Primer Design: Important Considerations and Tips for Good Primer Design Description: Running a PCR experiment and need to design primers? We all have been there. Primer design is essential for successful PCR, but where do you start? In this video, we’ll go through important considerations and tips for good primer design! Here are some helpful time stamps for our Primer Design video: 0:41 Primer Length 1:06 Considerations for Melting Temperature (Tm) and Annealing Temperature (Ta) 2:40 GC Content 3:16 Avoid Repeats in Your Primer Sequence 3:39 Avoid Complementary Sequences Within the Primer 4:06 Avoid Secondary Structures in Target Sequences 4:31 Summary 5:07 Avoid List For more information about abm's best primer design synthesis service, please visit: https://www.abmgood.com/Primer-Design-Synthesis.html Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences: ➜ Facebook: https://www.facebook.com/appliedbiological.materials/ ➜ Twitter: https://twitter.com/abm_good ➜ LinkedIn: https://www.linkedin.com/company/applied-biological-material ➜ Instagram: https://www.instagram.com/abm_good ➜ Blog: https://info.abmgood.com/blog </video>

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Subscribe to our channel for educational videos covering essential topics in cell biology and life sciences. From practical "How-to" videos for those ready to get started in the lab to videos introducing basic principles of important new scientific techniques. Send us your requests by posting a comment!

Applied Biological Materials (abm) provides quality research reagents and custom services at incredible prices. We have an extensive product range to make abm your one-stop supplier for all your research needs: PCR, RT-PCR, qPCR, antibodies, siRNA, miRNA, CRISPR, stable cell lines, immortalized cell lines, growth factors, and various viral expression systems (adenovirus, lentivirus, AAV). Conducting research shouldn’t cost you a fortune! abm is here to help!

→ Our company website: https://www.abmgood.com/
→ Our FREE learning resources: https://info.abmgood.com/learning-resources

#SciComm #CellBiology

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