Channel Title: The Ahmed Lab: Northwestern Neurosurgery Channel Description: The Ahmed Lab is a research laboratory at Northwestern Medicine's Department of Neurosurgery. We study glioblastoma, which is the most common type of adult malignant brain tumor. This channel is all about showing you what we do - from giving a snapshot of our everyday lives to creating teaching content on common skills and techniques in research. You can learn more about us at www.ahmed-lab.org or @ahmedlabnw (twitter). <video> MTT Assay for Cell Viability Description: Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video provides a detailed overview of how to perform an MTT cell viability assays. We discuss the theory behind the assay, the potential pitfalls, the step-by-step procedure, and the analysis and interpretation of these assays. All research shown here is from the Ahmed Lab, supported by NIH/NINDS funding. Further protocols can be found at ahmed-lab.org </video> <video> Welcome to Wet Lab: Pipetting & Other Basics Description: Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video provides an introduction to some basic wet lab concepts for any molecular biology laboratory. It includes a discussion of some general rules of thumb in the lab, understanding the different types of trash, different tubes and tools available, and finally goes through how to set and use various types of pipettes in the lab. </video> <video> Quantitative Polymerase Chain Reaction (qPCR) Description: Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 Full protocols for all assays available at ahmed-lab.org This video provides an overview of the theory and practice of quantitative PCR, a widely used technique in molecular biology laboratories in order to measure the mRNA expression of genes or to measure activation of gene transcription for specific genes. Here, we go through both the theory behind it and the practical aspects of getting primers, designing them appropriately, planning out your experiment, and interpreting and troubleshooting results. This work is coming from the Ahmed Lab at Northwestern supported by funding from the NIH/NINDS. </video> <video> Developing a Scientific Hypothesis Description: Created by Shivani Baisiwala, BS, MS, MD candidate 2021 This video is meant as a basic introduction to how to think about developing a scientific question, creating a hypothesis, and validating that hypothesis. It also briefly goes into how to build up a project from a hypothesis and how to think about validating specific mechanisms. All work shown here is supported by the Ahmed Lab at Northwestern University's Department of Neurosurgery, funded by the NIH and NINDS. </video> <video> Gene Modulation: Creating Knockdowns, Overexpressions, and Reporters Description: Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video provides a detailed overview of how to use plasmids to modulate gene expression in the lab. This technique is used for many research applications as it can help us directly link a gene expression change to a specific cell phenotype and can allow us to test novel gene therapies in the lab. We discuss here starting from a glycerol stock, what a plasmid is, how to expand it, and then how to use it to transfect or transduce cells to generate temporary or stable modifications respectively. We also take into account some key considerations and potential pitfalls when performing these assays and then end with a day-by-day walk through of how to do this in your lab. All research shown here is from the Ahmed Lab, supported by NIH/NINDS funding. Further protocols can be found at ahmed-lab.org </video> <video> Western Blot Protocol Overview Description: Created by Shivani Baisiwala, BS, MS, MD See protocols on www.ahmed-lab.org This video provides a step-by-step overview of the theory and practice of western blotting, which is a technique used in molecular biology to identify the levels of specific proteins of interest across multiple conditions. Here, we go through practical aspects of performing a blot but also discuss the reasoning behind each piece of the process. </video> <video> Intro to Proteomics / Mass Spectrometry (MS) Description: Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video covers the basics of how to setup and interpret a proteomics screen. All research in this video is performed at the Ahmed Lab at Northwestern's Department of Neurosurgery, and is supported by the NIH and NINDS. </video> <video> Immunofluorescence (IF), Immunohistochemistry (IHC), and Immunocytochemistry (ICC) Description: Created by Shivani Baisiwala, BS, MS Full protocols available on our website www.ahmed-lab.org This video covers the common technique of IF, which is also known as IHC or ICC depending on the type of sample being studied. This is a common and powerful technique used to study protein localization and expression in cells and tissues. All materials in this video are supported by the Ahmed Lab at Northwestern University's Department of Neurosurgery, funded by the NIH/NINDS. </video> <video> Introduction to Cell Culture Description: Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 See protocols on www.ahmed-lab.org This video provides an overview of the steps involved in passaging cells in order to maintain, count, harvest or seed them for future experiments. This is useful for many molecular biology assays as cells form the basis and must be consistently maintained in order to do assays. </video> <video> Introduction to R Description: Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video is intended as a basic introduction to performing analyses in R, directed at new users. All work shown here is performed at the Ahmed Lab at Northwestern Neurosurgery, funded by the NIH and NINDS. </video> <video> Ahmed Lab 2021 Description: The mission statement for the Ahmed Lab @ Northwestern Feinberg School of Medicine </video> <video> RNASeq Overview Description: Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video discusses a basic overview of RNA-Seq, explaining how it works and how it is typically analyzed and visualized. Any ongoing work presented here is performed by the Ahmed Lab at Northwestern University's Department of Neurosurgery, supported by NIH/NINDS. </video> <video> Writing a Scientific Project Proposal Description: Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This is a basic overview of how to write a project proposal. It is directed at a simple undergrad or early graduate proposal and gives an overview of key concepts to include. All work discussed or referenced in these videos is supported by the Ahmed Lab at Northwestern's Department of Neurosurgery and funded by NIH/NINDS grants. </video> <video> GBM Research: An Educational Series Description: Our channel focuses on providing high quality content about GBM research and broader molecular biology research topics. Subscribe for useful educational content! </video> <video> Flow Cytometry Description: Created by Shivani Baisiwala, BS, MS, MD Candidate 2021 This video provides a basic introduction to understanding how flow cytometry works, how it can be interpreted, and some key considerations in how to perform flow experiments. We discuss the fluorophores available and the different graphs and gatings that must be performed to assess the data. All research shown here is from the Ahmed Lab, supported by NIH/NINDS funding. Further protocols can be found at ahmed-lab.org </video>

Description

The Ahmed Lab is a research laboratory at Northwestern Medicine's Department of Neurosurgery. We study glioblastoma, which is the most common type of adult malignant brain tumor. This channel is all about showing you what we do - from giving a snapshot of our everyday lives to creating teaching content on common skills and techniques in research. You can learn more about us at www.ahmed-lab.org or @ahmedlabnw (twitter).

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