Channel Title: Amrita Vlab Channel Description: Virtual Lab at Amrita Intelligent development of virtual laboratories with state-of-the-art computer simulation technology to create real world environments and problem handling capabilities is required to bridge the gap between institutions (or industries) that retain the physical laboratory and distantly placed economically challenged educational institutions. Amrita Virtual Labs video channel is developed by Amrita CREATE, Amrita University. <video> Titration Curves of Aminoacids - Amrita University Description: ▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=3&brch=63&sim=1336&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu Alpha amino acids are the building blocks of proteins. Almost all proteins consist of various combinations of the same 20 amino acids. Amino acids are compounds containing both an amine group, -NH2, and a carboxylic acid group, -COOH. In addition there is an "R" group which is different for each amino acid. In physiological systems where the pH is near neutrality, the amino group of an amino acid will be protonated and the carboxylic acid group will be unprotonated. The acid-base behavior of amino acids is best described by Brønsted theory of acids and bases.A simple amino acid (that does not have an acid or base group in the "R" group) is a dibasic acid in its fully protonated form; it can donate two protons during its complete titration with a base. </video> <video> Latex Agglutination Test - Amrita University Description: ▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=3&brch=69&sim=195&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu Latex agglutination tests have been in use since 1956 t o detect a wide range of analytes in the clinical laboratory. When spectrophotometers and nephelometers are used in place of the human eye to detect agglutination, it is possible to measure quantitatively and to develop sensitive particle immunoassays. Latex particles may be build from different organic materials to a desired diameter, and may be functionalized with chemical groups to facilitate attachment of molecules Proteins and other molecules may be passively adsorbed to the latex particles or covalently coupled to functional groups. Some described automated latex agglutination tests have sensitivities of a few picograms of analyte. </video> <video> Amrita Vlab Live Stream Description: </video> <video> Maintenance and Storage of DH5 alpha E.coli Cells - Amrita University Description: ▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=3&brch=77&sim=1095&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu Escherichia coli is a rod-shaped bacterium with a circular chromosome about 3 million base pairs (bp) long. It can grow rapidly on minimal medium that contains a carbon compound such as glucose (which serves both as a carbon source and an energy source) and salts which supply nitrogen, phosphorus, and trace metals. E. coli grows more rapidly,however, on a rich medium that provides the cells with amino acids, nucleotide precursors,vitamins, and other metabolites that the cell would otherwise have to synthesize. Most strains of E. coli can be stored for years in stab vials, or indefinitely if frozen at −70°C. It is prudent to check the genetic markers of a strain revived from storage. Effective storage means that the organism is being maintained in a viable state without contamination and changes in genotypic or phenotypic characteristics. The organism must be easily restored to the same condition it was in prior to preservation. Several methods are available for the preservations of the microorganisms but there are two criteria for selecting a preservation method for a given culture. They are: i) period of preservation desired and ii) nature of culture to be preserved. For different physiological groups of bacteria, specific maintenance requirements exist. </video> <video> Estimation of Blood Glucose by Glucose Oxidase Method - Amrita University Description: ▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=3&brch=63&sim=1343&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu Enzymatic method yields maximum specificity for glucose estimation. Glucose can be measured by its reaction with glucose oxidase, in which gluconic acid and hydrogen peroxide are formed. Hydrogen peroxide than reacts with an oxygen acceptor, such as ortho-dianisidine,phenylamine-phenazone or any other chromogenic oxygen acceptors, in a reaction catalysed by peroxidase to form a colour. One of the advantages of enzymatic method is its specificity. This enables the estimation of glucose even in a complex mixture. Thus, this method is widely used in the field of clinical chemistry and for food analysis. In clinical chemistry, the enzymatic analysis of glucose in blood and urine has been modified as dipstick test. However in the present practical, the conventional enzymatic method shall be used. Advantages of Glucose Oxidase Method: 1. This method most specific enzymatic than other method such as; reduction of cupric to cuprous salts, and reduction of ferricyanide to ferrocyanide method. 2. The reaction can be monitored polarographically either by measuring the rate of disappearance of oxygen. </video> <video> Resistivity by Four Probe Method - Amrita University Description: ▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=1&brch=282&sim=1512&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu Resistivity and conductivity type are fundamental properties of semiconductors and are critical parameters in both materials research and wafer fabrication.The resistivity can affect a device's series resistance, threshold voltage, capacitance, and other parameters. Measuring a semiconductor material's resistivity is one of the most common electrical tests. four-point collinear probe and the appropriate test equipment can be used to determine both resistivity and conductivity type. The four-point, or Kelvin, probe method is the most common way to measure a semiconductor material's resistivity. Two of the probes are used to source current and the other two probes are used to measure voltage. Using four probes eliminates measurement errors due to the probe resistance, the spreading resistance under each probe, and the contact resistance between each metal probe and the semiconductor material. This technique involves bringing four equally spaced probes into contact with the material of unknown resistance. </video> <video> Preparation of Buffer stocks (TBE, TE and TAE) - Amrita University Description: ▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=3&brch=77&sim=1322&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu A buffer functions to resist changes in hydrogen ion concentration as a result of internal and environmental factors. Buffers consist of a weak acid (HA) and its conjugate base (A--) or a weak base and its conjugate acid. Weak acids and bases do not completely dissociate in water, and instead exist in solution as an equilibrium of dissociated and undissociated species. All buffers have an optimal pH range over which they are able to moderate changes in hydrogen ion concentration. Factors like temperature and concentration can greatly influence the pKa, and therefore, the pH range over which a buffer system is most effective. Careful preparation of buffers is important for successful and reproducible experiments. TAE buffer is a buffer solution containing a mixture of Tris base, acetic acid and EDTA. TBE or Tris/Borate/EDTA, is a buffer solution containing a mixture of Tris base, boric acid and EDTA. TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA In molecular biology, TBE and TAE buffers are often used in procedures involving nucleic acids, the most common being electrophoresis. </video> <video> Michelson Interferometer - Amrita University Description: ▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=1&brch=189&sim=1519&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu The Michelson interferometer is historically important for its use by Michelson and Morley in 1887 to provide experimental evidence against the theory of the luminiferous aether. Michelson subsequently used the interferometer to measure the length of the standard metre in terms of the wavelength of an atomic spectral line. The basic idea of how an interferometer works, is to split a beam of light into two beams; delay one with respect to the other, and then recombine them to observe their interference. </video> <video> Heat Transfer by Conduction - Amrita University Description: ▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=1&brch=194&sim=801&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu The basic mechanisms of heat transfer are generally considered to be conduction,convection,boiling,condensation and radiation. A net amount of heat is always transferred from the hotter body to the colder body. In conduction, heat is carried by means of collisions between rapidly moving molecules closer to the hot end of a body of matter and the slower molecules closer to the cold end. Conduction is poorest in gases because their molecules are relatively far apart and so interact less frequently than in solids and liquids. Metals are the best conductors of heat because some of their electrons are able to move about relatively freely and can interact frequently by collisions. </video> <video> Black Body Radiation: Determination of Stefan's Constant - Amrita University Description: ▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=1&brch=194&sim=548&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu All warm bodies emit electromagnetic radiation as a result of the thermal motion of the charged particles from which they are made. An object which absorbs all radiation incident on it is known as an "ideal blackbody". To explore radiation from objects at certain temperatures, commonly known as "blackbody radiation". In 1879 Josef Stefan found an empirical relationship between the power per unit area radiated by a blackbody and the temperature, which Ludwig Boltzmann derived theoretically a few years later. This relationship is the Stefan-Boltzmann law. </video> <video> Heat Transfer by Natural Convection - Amrita University Description: ▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=1&brch=194&sim=791&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu Heat transfer theory seeks to predict the energy transfer that takes place between material bodies as a result of temperature difference. This energy transfer is defined as heat. The three modes by which heat can be transferred from one place to another are conduction, convection and radiation. In natural convection, the fluid motion occurs by natural means such as buoyancy. Since the fluid velocity associated with natural convection is relatively low, the heat transfer coefficient encountered in natural convection is also low. Although most of the emphasis today in the electronics cooling community is devoted to extending forced convection cooling capability, many applications still depend upon natural convection cooling. Basically, natural convection cooling combined with radiation is what results when a fan is not used in the cooling design to move air. </video> <video> Mitosis in Onion Root Tip - Amrita University Description: ▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=3&brch=188&sim=1102&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu A process by which a parent cell divides into two or more daughter cells is called cell division. Cell division is actually a small part of the cell cycle. The type of cell division in normal eukaryotic cells is called Mitosis. Another type of cell division is also present in reproductive cells of eukaryotes is called meiosis. Cell cycle is mainly divided into two segments: Interphase and M phase. Interphase is a longer period of cell division. During this phase the cell prepares of its next stage. This is a period of diverse activities and these activities prerequisite for the next mitotic phase. Interphase is mainly divides into three phases: G1 phase, S phase and G2 phase. S phase is the period of replication. G1 and G2 are two gap phases during which the cell grows, producing proteins and preparing the cells. These phases also have certain check points and the whole cell cycle is strictly regulated. </video> <video> Tangent Galvanometer - Amrita University Description: ▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=1&brch=192&sim=1049&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu The earth exhibits a weak magnetic field at a given point on the earth's surface. This field has a horizontal component (parallel to the earth) and a vertical component (perpendicular to the earth). The instrument used in this experiment is a tangent galvanometer, to measure the horizontal component of the field, that consists of circular coils of wire oriented in a vertical plane. A magnetic field that is parallel to the earth's surface is thus produced. </video> <video> Qualitative Analysis of Amino Acids - Amrita University Description: ▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=3&brch=63&sim=1094&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu Analysis of amino acids is required in several areas of research, and it is a fundamental tool in product analysis. The application imposes different requirements on the analytical method because the amino acids play different roles. Amino acids are the basic constituents of proteins. Qualitative and quantitative analysis of the amino acid composition of hydrolyzed samples of pure proteins or peptides is used to identify the material and to directly measure its concentration Amino acids are also intermediates in metabolic pathways, often not directly involving proteins. The amino acids are measured as elements of physiological and nutritional studies. This has proven particularly important in monitoring the growth of cells in cultures, as used in the production of biopharmaceuticals . Similar considerations lead to the analysis of foods and feeds to ensure that nutritional requirements are met. </video> <video> Litmus Milk Test - Amrita University Description: ▶ This video channel is developed by Amrita University's CREATE http://www.amrita.edu/create ▶ Subscribe @ https://www.youtube.com/user/amritacreate http://www.youtube.com/amritavlab ▶ Like us @ https://www.facebook.com/CREATEatAmrita ▶ For more Information @ http://vlab.amrita.edu/index.php?sub=3&brch=76&sim=708&cnt=1 ▶ Amrita Virtual Lab Project website http://vlab.amrita.edu The Litmus Milk test is a rather complex test containing milk, litmus (a pH indicator), lactose, and casein (a milk protein) all of which can be metabolized by some bacteria. Litmus milk also contains sulfur and many nutrients that are found in milk. Litmus Milk has been used for many years for determining the metabolic activities of microorganisms in milk as an aid to the identification of bacterial species.It is especially useful in species differentiation within the genus Clostridium. This medium is also of value in the maintenance and propagation of lactic acid bacteria. For identification, organisms must be in pure culture. Morphological, biochemical, and/or serological tests should be performed for final identification </video>

Contacts and Links

Description

Virtual Lab at Amrita Intelligent development of virtual laboratories with state-of-the-art computer simulation technology to create real world environments and problem handling capabilities is required to bridge the gap between institutions (or industries) that retain the physical laboratory and distantly placed economically challenged educational institutions.

Amrita Virtual Labs video channel is developed by Amrita CREATE, Amrita University.

Videos from channel