Channel Title: Dr. Asif's Mol. Biology Channel Description: Hi, I am Dr. Asif Ali, a Molecular biologist by training and now working as a Postdoctoral Researcher. This channel aims to build a platform for fresh graduates, who are just starting their research and facing problems in Biotechnology and Molecular Biology. I will cover different topics of scientific writing skills, advanced laboratory equipment tutorials, and training in bioinformatics tools and software. If you have queries related to any video/research, please ask in the comments section of the relevant video, so that others can also benefit from this discussion. [🛑🛑🛑If you are stuck with RNA-seq data analysis or need my help for postdoc/scholarship application: Book a live session: see the below-given link🛑🛑🛑] <video> Top 5 R Packages used in Bioinformatics Description: #top5 #R #bioinformatics </video> <video> How to use a spectrophotometer? Description: #howtouse #spectrophotometer #asifmolbio Using a spectrophotometer involves careful steps to ensure accurate and reproducible results. Here’s a general guide: --- ### **1. Familiarize Yourself with the Instrument** - Understand the components of the spectrophotometer, including the light source, monochromator, sample holder, and detector. - Read the user manual specific to your spectrophotometer model. --- ### **2. Prepare the Instrument** 1. **Turn On the Spectrophotometer:** - Allow the lamp to warm up (usually 15–30 minutes) for consistent performance. 2. **Select the Wavelength:** - Set the spectrophotometer to the desired wavelength based on your experiment. --- ### **3. Prepare the Samples** 1. **Clean Cuvettes:** - Ensure the cuvettes are clean and free from scratches or fingerprints. - Use lint-free tissues to wipe the cuvette surfaces. 2. **Prepare Blank Solution:** - The blank should contain all components of your sample except the analyte (the substance to be measured). 3. **Prepare the Test Solutions:** - Dilute your samples to the required concentration. - Mix well to ensure homogeneity. --- ### **4. Calibrate the Instrument** 1. **Insert the Blank:** - Place the blank solution in the cuvette holder. - Close the lid to prevent light interference. 2. **Set the Baseline:** - Adjust the spectrophotometer to read **0% absorbance** or **100% transmittance**. --- ### **5. Measure the Sample** 1. **Insert the Sample:** - Replace the blank with the sample solution in the same orientation to ensure consistent readings. 2. **Record the Reading:** - Note the absorbance or transmittance displayed on the spectrophotometer. 3. **Repeat for Multiple Samples:** - Follow the same procedure for each sample to ensure uniformity. --- ### **6. Analyze Data** - Use the absorbance readings and apply Beer-Lambert’s law, if applicable, to determine the concentration of the analyte. --- ### **7. Clean and Store** - Rinse cuvettes with distilled water immediately after use to prevent residue buildup. - Turn off the spectrophotometer and cover it to protect it from dust. --- ### **Tips for Accurate Measurements** - Always use matched cuvettes (same material and dimensions) for all readings. - Avoid bubbles in the cuvette as they can scatter light. - Handle cuvettes only by the frosted sides to avoid smudging the optical path. </video> <video> Pre-load folder unavailable for selection in data upload to NCBI Description: #ncbi #data #notworking #problemsolved In this video, I assisted a student in resolving an issue where the 'preload' folder was unavailable for selection during a FileZilla data upload to NCBI. After troubleshooting, we identified the cause of the issue and walked through the steps needed to ensure the correct folder was accessible for a smooth upload process. → Learn R with Dr. Asif. Biology: • Learn R with Dr. Asif → Tips to improve Science Writing skills : • Tips to improve Science Writing skills → Designing plots with web-based tools: • Designing plots with web-based tools → Interpretation of Transcriptomics, Proteomics and metabolomics : → Phylogenetic analysis: • Phylogenetic analysis → Experiment Tutorials: • Hands on performing experimental skills ★If you have queries related to any video/research, please ask in the comments section of the relevant video, so that others can also get benefit from this discussion. [🛑🛑🛑If you are stuck with RNA-seq data analysis or need my help for postdoc/scholarship application: Book a live session: see the below-given link🛑🛑🛑] ★🔴 IMPORTANT LINKS 🔴★ → Book a session with Dr. Asif: forms.gle/aEVUtAzrbSHxeN659 → Facebook: / drasifmolbiology → Twitter: / asifmolbio → Instagram: / asifmolbio → ORCID: https://orcid.org/0000-0001-7688-148X </video> <video> How to draw and interpret the Partial Mantel's test? Description: #howtodraw #interpretation #mantel's #asifmolbio In this video, I demonstrate how to interpret the results of the Mantel test, which assesses the correlation between two distance matrices. I also explain how to draw and visualize the Mantel test results, highlighting their significance in understanding relationships between genetic and environmental data. *******R_script and example files download ; https://github.com/asifmolbio/Mantel-s-test********* → Learn R with Dr. Asif. Biology: • Learn R with Dr. Asif → Tips to improve Science Writing skills : • Tips to improve Science Writing skills → Designing plots with web-based tools: • Designing plots with web-based tools → Interpretation of Transcriptomics, Proteomics and metabolomics : → Phylogenetic analysis: • Phylogenetic analysis → Experiment Tutorials: • Hands on performing experimental skills ★If you have queries related to any video/research, please ask in the comments section of the relevant video, so that others can also get benefit from this discussion. [🛑🛑🛑If you are stuck with RNA-seq data analysis or need my help for postdoc/scholarship application: Book a live session: see the below-given link🛑🛑🛑] ★🔴 IMPORTANT LINKS 🔴★ → Book a session with Dr. Asif: forms.gle/aEVUtAzrbSHxeN659 → Facebook: / drasifmolbiology → Twitter: / asifmolbio → Instagram: / asifmolbio → ORCID: https://orcid.org/0000-0001-7688-148X </video> <video> How to perform WGCNA (Weighted Gene Co-expression Network Analysis using R Studio? Description: #howtoperform #wgcna #coexpression #network In this video, I have provided a complete R script to perform GCNA (Weighted Gene Co-expression Network Analysis using R studio. GitHub link ★ https://github.com/asifmolbio/WGCNA → Learn R with Dr. Asif. Biology: • Learn R with Dr. Asif → Tips to improve Science Writing skills : • Tips to improve Science Writing skills → Designing plots with web-based tools: • Designing plots with web-based tools → Interpretation of Transcriptomics, Proteomics and metabolomics : → Phylogenetic analysis: • Phylogenetic analysis → Experiment Tutorials: • Hands on performing experimental skills ★If you have queries related to any video/research, please ask in the comments section of the relevant video, so that others can also get benefit from this discussion. [🛑🛑🛑If you are stuck with RNA-seq data analysis or need my help for postdoc/scholarship application: Book a live session: see the below-given link🛑🛑🛑] ★🔴 IMPORTANT LINKS 🔴★ → Book a session with Dr. Asif: forms.gle/aEVUtAzrbSHxeN659 → Facebook: / drasifmolbiology → Twitter: / asifmolbio → Instagram: / asifmolbio → ORCID: https://orcid.org/0000-0001-7688-148X </video> <video> How to make a powerpoint presentation of a research proposal? Description: #howtomake #powerpoint #presentation #researchproposal In this video, I provide key tips and practical examples for preparing a presentation of your research proposal. From structuring your slides to effectively showcasing each component of the proposal, I guide you through crafting a clear, engaging, and professional presentation. You'll learn how to present your introduction, objectives, methodology, and expected outcomes in a concise and visually appealing way. By the end of the video, you'll be equipped with the tools to confidently deliver a presentation that captures your audience's attention and effectively communicates your research plan. Learn R with Dr. Asif. Biology: https://www.youtube.com/playlist?list=PL3fbGvDVm3utgWIqRiGnuZ8g2GcV5Pe6B Tips to improve Science Writing skills: https://www.youtube.com/playlist?list=PL3fbGvDVm3uttBxDhdT5f-0hZqlDWSYkP Designing plots with web-based tools: https://www.youtube.com/playlist?list=PL3fbGvDVm3uvivuNiZN_v-mWj8JbAsQX2 Interpretation of Transcriptomics, Proteomics and metabolomics: https://www.youtube.com/playlist?list=PL3fbGvDVm3uspnyI1_RVNtdPDyvInHh1t Phylogenetic analysis: https://www.youtube.com/playlist?list=PL3fbGvDVm3usnd3Qidi5eX8azL-BUbZu2 Experiment Tutorials: https://www.youtube.com/playlist?list=PL3fbGvDVm3uuOop_xX6mrWDPAE5T8_4Hu If you have queries related to any video/research, please ask in the comments section of the relevant video, so that others can also get benefit from this discussion. [🛑🛑🛑If you are stuck with RNA-seq data analysis or need my help for postdoc/scholarship application: Book a live session: see the below-given link🛑🛑🛑] 🔴 IMPORTANT LINKS 🔴 Book a session with Dr. Asif: forms.gle/aEVUtAzrbSHxeN659 Facebook: https://www.facebook.com/drasifmolbiology Twitter: https://twitter.com/asifmolbio Instagram: https://www.instagram.com/asifmolbio/ ORCID: https://orcid.org/0000-0001-7688-148X 0:00 Intro 3:39 Title 4:56 Introduction 10:46 Literature Review 14:58 Research Objectives 16:07 Methodology 18:59 Significance of the Study 19:47 Expected Outcome 20:31 Timeline 22:20 Tips to impress audience 24:42 Tips to improve formatting </video> <video> How to Master PowerPoint Presentation Skills? Description: #howto #mastering #powerpoint #presentation Mastering PowerPoint presentation skills can significantly enhance your ability to communicate your ideas effectively. Here are some key tips to help you improve: ### 1. **Start with a Clear Structure** - **Outline Your Presentation**: Before diving into slides, outline your content. Make sure you have a clear introduction, body, and conclusion. - **Stick to One Message Per Slide**: Each slide should focus on a single point to avoid overwhelming your audience. ### 2. **Design for Clarity** - **Use Simple, Clean Layouts**: Avoid clutter. A clean design with clear fonts, sufficient white space, and simple graphics will make your message more digestible. - **Consistent Theme**: Use a consistent theme throughout. PowerPoint offers various templates, but ensure colors, fonts, and visual elements are uniform. - **High-Quality Visuals**: Use high-resolution images, graphs, and diagrams. Visuals should enhance the content, not distract from it. ### 3. **Effective Use of Text** - **Keep Text Minimal**: Use bullet points and short phrases. The audience should be listening to you, not reading the slides. - **Large, Readable Fonts**: Use easy-to-read fonts (like Arial or Calibri) with a font size of at least 24 for main text and larger for titles. - **Contrast**: Ensure high contrast between text and background, making it readable from a distance. ### 4. **Use Visuals and Data Effectively** - **Graphs and Charts**: Use graphs to present data, but ensure they are simple and easy to understand. Label axes and use legible numbers. - **Images and Icons**: Only use images that are relevant to your message. Too many can make the slide feel cluttered. - **Animations**: Use animations sparingly. Simple fades or transitions can help, but excessive use can become distracting. ### 5. **Engage the Audience** - **Tell a Story**: Structure your presentation like a story—set up a problem, show the journey, and provide a resolution. - **Ask Questions**: Involve the audience by asking questions or inviting feedback. This keeps them engaged. - **Body Language**: Maintain good posture and make eye contact. Avoid standing behind the podium; move around to engage with the audience. ### 6. **Practice** - **Rehearse**: Practice your presentation several times. This will help you become comfortable with the flow and timing. - **Use Speaker Notes**: You can use the speaker notes section to jot down key points. This keeps you on track without cluttering your slides. - **Time Your Presentation**: Ensure your presentation fits within the allotted time. Too long, and you risk losing your audience's attention. ### 7. **Master Transitions and Animations** - **Simple Transitions**: Stick to simple transitions between slides (e.g., "Fade" or "Push") to avoid distracting the audience. - **Animations for Emphasis**: Use animations (like "Appear" or "Zoom") to highlight key points, but don't overdo it. ### 8. **Use Audio and Video** - **Embed Videos**: If you need to show a video, embed it into your presentation, rather than linking to an external file. This ensures everything works smoothly. - **Audio**: Use audio files if they enhance your message, such as a brief introduction or background sound, but ensure it's of good quality. ### 9. **Be Prepared for Technical Issues** - **Check Equipment**: Before presenting, ensure the projector, laptop, and remote are working properly. - **Backup Plan**: Have a backup plan for technical issues (e.g., printouts or an alternate device to present from). ### 10. **End Strong** - **Summarize Key Points**: Conclude with a recap of the most important takeaways. - **Call to Action**: If appropriate, include a call to action (e.g., ask the audience to get involved, visit a website, etc.). - **Questions and Feedback**: Leave time for questions at the end of the presentation. By focusing on these elements, you can ensure that your PowerPoint presentations are clear, engaging, and memorable. </video> <video> Using ChatGPT to improve English language in research writing? Description: #chatgpt #englishlanguage #research In this video, I have addressed if can we use generative AI models like ChatGPT to polish manuscripts? → Learn R with Dr. Asif. Biology: • Learn R with Dr. Asif → Tips to improve Science Writing skills : • Tips to improve Science Writing skills → Designing plots with web-based tools: • Designing plots with web-based tools → Interpretation of Transcriptomics, Proteomics and metabolomics : → Phylogenetic analysis: • Phylogenetic analysis → Experiment Tutorials: • Hands on performing experimental skills ★If you have queries related to any video/research, please ask in the comments section of the relevant video, so that others can also get benefit from this discussion. [🛑🛑🛑If you are stuck with RNA-seq data analysis or need my help for postdoc/scholarship application: Book a live session: see the below-given link🛑🛑🛑] ★🔴 IMPORTANT LINKS 🔴★ → Book a session with Dr. Asif: forms.gle/aEVUtAzrbSHxeN659 → Facebook: / drasifmolbiology → Twitter: / asifmolbio → Instagram: / asifmolbio → ORCID: https://orcid.org/0000-0001-7688-148X </video> <video> How to create an audio podcast of a research article using SciSpace? Description: #howtocreate #podcast #scispace #ai In this video, I have tested the podcast feature of SciSpace **********LINK and REFERRAL CODES*********** https://typeset.io/?via=asif For Indian users! ASIF20 — offers 20% off on monthly plan ASIF10 — offers 10% off on annual plan For Other countries: AF20 — offers 20% off on monthly plan AF40 — offers 40% off on annual plan → Learn R with Dr. Asif. Biology: • Learn R with Dr. Asif → Tips to improve Science Writing skills : • Tips to improve Science Writing skills → Designing plots with web-based tools: • Designing plots with web-based tools → Interpretation of Transcriptomics, Proteomics and metabolomics : → Phylogenetic analysis: • Phylogenetic analysis → Experiment Tutorials: • Hands on performing experimental skills ★If you have queries related to any video/research, please ask in the comments section of the relevant video, so that others can also get benefit from this discussion. [🛑🛑🛑If you are stuck with RNA-seq data analysis or need my help for postdoc/scholarship application: Book a live session: see the below-given link🛑🛑🛑] ★🔴 IMPORTANT LINKS 🔴★ → Book a session with Dr. Asif: forms.gle/aEVUtAzrbSHxeN659 → Facebook: / drasifmolbiology → Twitter: / asifmolbio → Instagram: / asifmolbio → ORCID: https://orcid.org/0000-0001-7688-148X </video> <video> Article forage : An AI tool to create content on any topic Description: #content #ai #article #writing </video> <video> What is the difference between omics and phenomics Description: #omics #phenomics #difference </video> <video> Facts you did know before Description: #facts #youdidntknow #mountains </video> <video> Copy AI writing tool for academic needs Description: #copy #AI #tools #writing #academic </video> <video> Data submission to sequence read archive (SRA) NCBI Description: #howtosubmit #SRA #NCBI #data In this video, I provide a step-by-step guide on how to submit your sequencing data to the Sequence Read Archive (SRA) at NCBI. You'll learn how to prepare and upload your data, ensuring proper metadata submission. This tutorial is perfect for researchers looking to archive their sequencing datasets in compliance with NCBI’s submission standards. Genome-Wide analysis: https://www.youtube.com/playlist?list=PL3fbGvDVm3usUlYa1EYeck6b4j4-hOSGb Learn R with Dr. Asif's Mol. Biology: https://www.youtube.com/playlist?list=PL3fbGvDVm3utgWIqRiGnuZ8g2GcV5Pe6B Scientific writing Skills: https://www.youtube.com/playlist?list=PL3fbGvDVm3uttBxDhdT5f-0hZqlDWSYkP Web-based tools and concepts: https://www.youtube.com/playlist?list=PL3fbGvDVm3uvivuNiZN_v-mWj8JbAsQX2 Transcriptomics & Proteomics : https://www.youtube.com/playlist?list=PL3fbGvDVm3uspnyI1_RVNtdPDyvInHh1t Phylogenetic analysis: https://www.youtube.com/playlist?list=PL3fbGvDVm3usnd3Qidi5eX8azL-BUbZu2 Experiment Tutorials: https://www.youtube.com/playlist?list=PL3fbGvDVm3uuOop_xX6mrWDPAE5T8_4Hu If you have queries related to any video/research, please ask in the comments section of the relevant video, so that others can also get benefit from this discussion. [🛑🛑🛑If you are stuck with RNA-seq data analysis or need my help for postdoc/scholarship application: Book a live session: see the below-given link🛑🛑🛑] 🔴 IMPORTANT LINKS 🔴 Book a session with Dr. Asif: forms.gle/aEVUtAzrbSHxeN659 Facebook: https://www.facebook.com/drasifmolbiology Twitter: https://twitter.com/asifmolbio Instagram: https://www.instagram.com/asifmolbio/ ORCID: https://orcid.org/0000-0001-7688-148X </video> <video> Top most used AI Chrome extensions for academic needs Description: #asifmolbio #AI #chrome #ai Top most used AI Chrome extensions for academic needs 1. Fireflies.ai: Records and summarizes meetings. 2. Speak AI: Transcribes and analyzes media. 3. Otter.ai: Real-time transcription and notes. 4. Gizzmo: Automates workflows. 5. NaturalReader: Converts text to speech. 6. Paragraph AI: Enhances and generates text. </video>

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Description

Hi, I am Dr. Asif Ali, a Molecular biologist by training and now working as a Postdoctoral Researcher.
This channel aims to build a platform for fresh graduates, who are just starting their research and facing problems in Biotechnology and Molecular Biology. I will cover different topics of scientific writing skills, advanced laboratory equipment tutorials, and training in bioinformatics tools and software.

If you have queries related to any video/research, please ask in the comments section of the relevant video, so that others can also benefit from this discussion.


[🛑🛑🛑If you are stuck with RNA-seq data analysis or need my help for postdoc/scholarship application: Book a live session: see the below-given link🛑🛑🛑]

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