Channel Title: Microcourses Channel Description: We are a team of light microscopists from core facilities at Harvard Medical School. We teach microscopy at HMS, and run the Quantitative Imaging: From Acquisition to Analysis course at Cold Spring Harbor Laboratory. On this channel, you can find practical instructional videos on various microscopy topics, pretty movies collected on microscopes in our cores, and some random fun stuff. Enjoy! This channel was created by Jennifer Waters, Director of the Core for Imaging Technology & Education (CITE) at Harvard Medical School & Chan Zuckerberg Initiative Imaging Scientist. (@JenCWaters on X & Bluesky) The Microcourses logo was designed by Eva de la Serna, Advanced Microscopy Fellow at Harvard Medical School (@sci_doodles on X & Bluesky). Links to our websites below <video> Spherical Aberration Description: Spherical aberration distorts images generated with a light microscope. In this Microcourse, you'll learn what spherical aberration is, what is does to your images, where it comes from and how to deal with it. Thanks to my fabulous group for feedback on this video prior to release: Talley Lambert, Anna Jost, Rylie Walsh & Federico Gasparoli. References & Resources: Arimoto, R. and Murray, J.M. (2004) A common aberration with water-immersion objective lenses. J. Microsc. 216, 49–51 Goodwin, P.C. (2013) Evaluating optical aberrations using fluorescent microspheres: methods, analysis, and corrective actions. Methods Cell Biol. 114, 369–385 Hell, S. et al. (2011) Aberrations in confocal fluorescence microscopy induced by mismatches in refractive index. J. Microsc. 169, 391–405 Hiraoka, Y. et al. (1990) Determination of three-dimensional imaging properties of a light microscope system. Partial confocal behavior in epifluorescence microscopy. Biophys. J. 57, 325–333 Joglekar, A.P. et al. (2008) Counting kinetochore protein numbers in budding yeast using genetically encoded fluorescent proteins. Methods Cell Biol. 85, 127–151 Ross, S.T. et al. (2014) Practical considerations of objective lenses for application in cell biology. Methods Cell Biol. 123, 19–34 Waters, J.C. (2007) Live‐Cell Fluorescence Imaging. Methods Cell Biol. 81, 115–140 Fluorescence mounting media suggestions: https://nic.med.harvard.edu/resources/media/ Chapters: 00:00 Introduction to spherical aberration 02:11 Identifying spherical aberration 02:48 Effects on image quality 04:36 Causes of spherical aberration: Optics 06:07 Causes of spherical aberration: Specimens 08:34 Why specimens cause spherical aberration 12:22 Spherical aberration with depth 12:51 Effect on intensity 14:00 Solutions for spherical aberration 14:38 Easier solutions 19:06 More complicated solutions 20:34 All solutions are limited </video> <video> Segmentation & Colocalization Description: Lecture by Cayla Miller at the Hands-on Quantitative Confocal Microscopy Workshop at the Salk Institute in August 2022 </video> <video> Detectors & Digital Imaging Description: Lecture by Talley Lambert (Lecturer in Systems Biology, Harvard Medical School) at the Hands-on Quantitative Confocal Microscopy Workshop at the Salk Institute in August 2022 </video> <video> Photobleaching Description: Anna Jost (Harvard Medical School), for Microtutor (microtutor.globalbioimaging.org) </video> <video> Trackmate Introduction and Demo Description: Lecture by Cara Schiavon at the Hands-on Quantitative Confocal Microscopy Workshop at the Salk Institute in August 2022 </video> <video> Optimizing Signal to Noise Ratio Description: Lecture by Rylie Walsh (Advanced Microscopy Fellow at Harvard Medical School) and Jennifer Waters (Director of the Nikon Imaging Center at Harvard Medical School & Chan Zuckerberg Initiative Imaging Scientist) at the Hands-on Quantitative Confocal Microscopy Workshop at the Salk Institute in August 2022 </video> <video> Fluorophore Brightness Description: Anna Jost (Harvard Medical School), for Microtutor (microtutor.globalbioimaging.org) </video> <video> Fundamental Concepts in Quantitative Microscopy Description: Lecture by Jennifer Waters (Director of the Nikon Imaging Center at Harvard Medical School & Chan Zuckerberg Initiative Imaging Scientist) at the Hands-on Quantitative Confocal Microscopy Workshop at the Salk Institute in August 2022 </video> <video> What is Microtutor LIVE? Description: Visit https://microtutor.globalbioimaging.org/ to learn more! </video> <video> Introduction to Colocalization in Fluorescence Microscopy Description: Dr. Federico Gasparoli is a Research Associate in the Image Analysis Collaboratory at Harvard Medical School. This lecture on co-localization was part of the first Microtutor LIVE Fluorescence Microscopy flipped-classroom course in Fall 2024. Learn more about Microtutor at https://microtutor.globalbioimaging.org/ </video> <video> Fluorophore Types & Labeling Methods Description: Anna Jost (Harvard Medical School), for Microtutor (microtutor.globalbioimaging.org) </video> <video> Imaging Live Specimens Description: Lecture by Jennifer Waters (Director of the Nikon Imaging Center at Harvard Medical School & Chan Zuckerberg Initiative Imaging Scientist) at the Hands-on Quantitative Confocal Microscopy Workshop at the Salk Institute in August 2022 </video> <video> Fluorophore Spectra Description: Anna Jost (Harvard Medical School), for Microtutor (microtutor.globalbioimaging.org) </video> <video> Microtutor: A new virtual light microscopy course Description: </video> <video> Confocal Microscopy Description: Lecture by Uri Manor (Assistant Research Professor & Director of the Waitt Advanced Biophotonics Core at the Salk Institute, & Chan Zuckerberg Initiative Imaging Scientist) at the Hands-on Quantitative Confocal Microscopy Workshop at the Salk Institute in August 2022 </video>

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Description

We are a team of light microscopists from core facilities at Harvard Medical School. We teach microscopy at HMS, and run the Quantitative Imaging: From Acquisition to Analysis course at Cold Spring Harbor Laboratory. On this channel, you can find practical instructional videos on various microscopy topics, pretty movies collected on microscopes in our cores, and some random fun stuff. Enjoy!

This channel was created by Jennifer Waters, Director of the Core for Imaging Technology & Education (CITE) at Harvard Medical School & Chan Zuckerberg Initiative Imaging Scientist. (@JenCWaters on X & Bluesky)

The Microcourses logo was designed by Eva de la Serna, Advanced Microscopy Fellow at Harvard Medical School (@sci_doodles on X & Bluesky).


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